Evaluation of mineral mixture, Probiotics, enzymes and acidifiers effect on serum biochemistry of broiler chickens

Abstract

The present study was conducted to evaluate the effects of dietary supplementation with  organic mineral mixtures, probiotics, enzymes, and acidifiers on the serum biochemical  parameters of broiler chickens. A total of 150 day-old straight-run VenCobb broiler  chicks were randomly assigned to five treatment groups (T0–T4) with three replicates  of 10 chicks each. The groups included a control (T0) receiving only a basal diet, while  T1 to T4 were supplemented with organic mineral mixture (0.50 g/kg), probiotics (1.0  g/kg), enzymes (0.50 g/kg), and acidifiers (1.0 g/kg), respectively. The birds were reared  under uniform management conditions for 42 days. At the end of the trial, serum  samples were analyzed for glucose, total cholesterol, triglycerides, urea, creatinine,  and BUN/creatinine ratio using commercial diagnostic kits. The results revealed a  significant reduction (p ≤ 0.05) in serum glucose, cholesterol, and triglyceride levels  in all supplemented groups compared to the control. The enzyme-supplemented group  (T3) exhibited the most pronounced reduction in lipid parameters. Urea and creatinine  concentrations were significantly lower in the supplemented groups, with the lowest  creatinine observed in T3, indicating improved protein metabolism and renal function.  The BUN/creatinine ratio was also significantly reduced in all treatment groups,  reflecting enhanced nitrogen utilization. These findings suggest that supplementation  with organic minerals, probiotics, enzymes, and acidifiers positively modulate the  serum biochemical profile of broiler chickens, indicating improved metabolic efficiency  and physiological health.