Antimicrobial effects of grapefruit seed extract microfibers  against mouse hepatitis virus, Escherichia coli,  Staphylococcus aureus, and Pseudomonas aeruginosa

Kazuo  Goto; Mitsuo Kiriya; Sillow Kakuma; Kazunori Horikoshi; Koichi  Suzuki

doi:10.48165/jlas.2024.7.1.1

Authors

Kazuo Goto Teikyo University Faculty of Clinical Laboratory Medicine, 2-11-1, Kaga Itabashi-ku, Tokyo 173-8605, Japan Author
Mitsuo Kiriya Teikyo University Faculty of Clinical Laboratory Medicine, 2-11-1, Kaga Itabashi-ku, Tokyo 173-8605, Japan Author
Sillow Kakuma Agenes inc., 1-6-17 Tamadaira, Hino, Tokyo 191-0062, Japan Author
Kazunori Horikoshi Agenes inc., 1-6-17 Tamadaira, Hino, Tokyo 191-0062, Japan Author
Koichi Suzuki Teikyo University Faculty of Clinical Laboratory Medicine, 2-11-1, Kaga Itabashi-ku, Tokyo 173-8605, Japan Author

DOI:

https://doi.org/10.48165/jlas.2024.7.1.1

Keywords:

Antimicrobial, disinfectant, grapefruit seed extract, microfiber

Abstract

Grapefruit seed extract (GSE), which contains flavonoids, possesses antibacterial, antiviral, and antifungal properties. This study investigated the antimicrobial activity of cellulose-bound GSE microfibers against mouse hepatitis virus (MHV) for three weeks and against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa for one week. The disinfectant was smeared on a petri dish to inactivate the MHV for three weeks or to prevent the growth of the selected bacteria for one week. As the disinfectant effect lasted at least one week, GSE microfibers could have applications in disinfecting laboratory animal facilities. The virus or bacteria mixed with the disinfectant did not cause cytopathic effects in the cells or growth on the media after one week.

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