Carry-Over Rate of Aflatoxin B1 from Feed to Milk and its Impact on Haemato-Biochemical Parameters in Nili-Ravi Buffaloes (Bubalus bubalis)
DOI:
https://doi.org/10.48165/ijvsbt.21.6.22Keywords:
Aflatoxin, Carry-over rate, Haemato-biochemical parameters, Milk safety, Nili-Ravi buffaloAbstract
The present study was undertaken to establish the transmission/carry-over rate of aflatoxin B1 (AFB1) from contaminated feed to AFM1 in milk of Nili-Ravi buffaloes. Twenty lactating buffaloes of almost similar weight (450-550 kg), lactation number (3-6) in early lactation (1-4 months) were divided into 5 treatments (T1 to T5), each having four replicates.The AFB1 concentration in feed of T1 to T5 was 0, 28, 56, 84 and 113 ppb, respectively. The average milk yield (kg/d) in treatments T1 to T5 during the collection period of experiment was 9.31±0.04, 9.24±0.04, 9.35±0.06, 9.42±0.07 and 9.42±0.05; and the total AFM1 excretion (µg/animal/5d) was 0.00±0.00, 19.20±0.04, 29.85±0.23, 39.00±0.14 and 47.74±0.14, respectively. The results revealed that the total AFM1 excretion increased with increasing concentration of AFB1 in feed. The average AFM1 concentration in T1 to T5 was 0.00±0.00, 0.41±0.01, 0.63±0.01, 0.83±0.01 and 1.01±0.01 ppb, respectively. The carry-over (%) of aflatoxin, i.e., transmission from feed to milk was 0.00, 0.76, 0.59, 0.51 and 0.48 in groups T1 to T5, respectively. The biochemical and haematological values in various treatment groups revealed that erythrocytes, PCV, haemoglobin and total protein content decreased (p<0.05); andGOT, GPT, GGT and ALP values increased (p<0.05) with increasing level of aflatoxin contamination in feed.The overall carry-over rate of aflatoxin in Nili-Ravi buffaloes was 0.59%, which might impose a health risk to the consumer. It was concluded that the daily intake of AFB1 in Nili-Ravi buffalo should not exceed 500 µg or 28 ppb in the total dry matter intake to keep the AFM1 content below permissible level.Downloads
References
Alonso, V.A., Monge, M.P., Larriestra, A., Dalcero, A.M., Cavaglieri, L.R., & Chiacchiera, S.M. (2010). Naturally occurring aflatoxin M1 in raw bulk milk from farm cooling tanks in Argentina. Food Additives & Contaminants: Part A, 27, 373–379.
Auerbach, H., Maas, R.F.M., Op den Camp, H.J.M., Pol, A., & Fink Gremmels, J. (1998). Biodegradation of aflatoxin B1 by bovine rumen micro-organism in vitro and its effects on rumen fermentation. Revue de Médecine Vétérinaire, 149, 573.
Bantaokul, C., & Ruangwises, S. (2010). Carry-over rate of aflatoxin M1 into cow milk during early lactation period. 9th Chulalongkorn University Veterinary Annual Conference, Bangkok, Thailand (Abstr. 34).
Battacone, G., Nudda, A., Cannas, A., Cappio, B.A., Bomboi, G., & Pulina, G. (2003). Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1. Journal of Dairy Science, 86, 2667–2675.
Bertoni, G., Trevisi, E., Bani, P., & Xingtai, H. (2001). Behaviour of digesta passage rate during lactation in primiparous and multiparous dairy cows. Proceedings of the ASPA XIV Congress, p. 144–146.
Bingol, N.T., Tanritanir, P., Dede, S., & Ceylan, E. (2007). Influence of aflatoxin present in forages and concentrated feeding stuffs on milk and some serum biochemical parameters in goats. Bulletin of the Veterinary Institute in Pulawy, 51, 65–69.
Britzi, M., Friedman, S., Miron, J., Solomon, R., Cuneah, O., & Shimshoni, J.A. (2013). Carry-over of aflatoxin B1 to aflatoxin M1 in high-yielding Israeli cows in mid- and late-lactation. Toxins, 5, 173–183.
Creppy, E.E. (2002). Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicology Letters, 127, 19–28.
Diaz, D.E., Hagler Jr, W.M., Blackwelder, J.T., Eve, J.A., Hopkins, B.A., Anderson, K.L., Jones, F.T., & Whitlow, L.W. (2004). Aflatoxin binders II: Reduction of aflatoxin M1 in milk by sequestering agents of cows consuming aflatoxin in feed. Mycopathologia, 157, 233–241.
FSSAI (2020). Manual of Methods of Analysis of Foods - Mycotoxins. Food Safety and Standards Authority of India, Ministry of Health and Family Welfare.
Huang, S., Zheng, N., Fan, C., Cheng, M., Wang, S., Jabar, A., Wang, J.Q., & Cheng, J.B. (2018). Effects of aflatoxin B1 combined with ochratoxin A and/or zearalenone on metabolism, immune function, and antioxidant status in lactating dairy goats. Asian Australasian Journal of Animal Sciences, 31, 505–513.
Huff, W.E., Kubena, R.B., Harvey, R.H., Corrier, D.E., & Mollenhauer, H.H. (1986). Progression of aflatoxicosis in broiler chickens. Poultry Science, 65, 1891–1899.
IARC (2002). Aflatoxins. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. IARC Press, Vol. 82, pp. 171–300.
Liu, Y., & Wu, F. (2010). Global burden of aflatoxin-induced hepatocellular carcinoma: A risk assessment. Environmental Health Perspectives, 118, 818–824.
Luna-Lopez, M.C., Valdivia-Flores, A.G., Rangel-Muñoz, E.J., Hernández-Valdivia, E., Quezada-Tristán, T., Jaramillo-Juárez, F., & Ortiz-Martínez, R. (2025). Time-dependent changes in performance, biochemistry, and histology in dairy calves with acute aflatoxicosis. Veterinary Sciences, 12, 273.
Masoero, F., Gallo, A., Moschini, M., Piva, G., & Diaz, D. (2007). Carryover of aflatoxin from feed to milk in dairy cows with low and high somatic cell counts. Animal, 1, 1344–1350.
Michlig, N., Signorini, M., Gaggiotti, M., Chiericatti, C., Basílico, J.C., Repetti, M.R., & Beldomenico, H.R. (2016). Risk factors associated with the presence of aflatoxin M1 in raw bulk milk from Argentina. Food Control, 64, 151–156.
Moschini, M., Gallo, A., Piva, G., & Masoero, F. (2008). The effects of rumen fluid on the in vitro aflatoxin binding capacity of different sequestering agents and in vivo release of the sequestered toxin. Animal Feed Science and Technology, 147, 292–309.
Moschini, M., Masoero, F., Diaz, D.E., Gallo, A., Pietri, A., & Piva, G. (2006). Plasma aflatoxin concentrations over time in bolus-fed lactating dairy cows. Journal of Animal Science, 84(Suppl. 1), 74.
Moschini, M., Masoero, F., Gallo, A., & Diaz, D. (2007). Mucosal absorption of aflatoxin B1 in lactating dairy cows. Italian Journal of Animal Science, 6(Suppl. 1), 324–326.
Mughal, M.J., Peng, X., Zhou, Y., & Fang, J. (2017). Aflatoxin B1 invokes apoptosis via death receptor pathway in hepatocytes. Oncotarget, 8(5), 8239–8249.
Naseer, O., Khan, J.A., Khan, M.S., Omer, M.O., Naseer, J., Sohail, M.L., Saleem, M.U., Ahmad, W., & Ahmad, A.S. (2017). Effect of dietary aflatoxins (AFB1) on hematological and biochemical indices of male buffaloes. Indian Journal of Animal Research, 52, 718–723.
Pons, W.A., Cucullu, A.F., Lee, L.S., Robertson, J.A., Franz, A.O., & Goldblatt, L.A. (1966). Determination of aflatoxins in agricultural products: Use of aqueous acetone for extraction. Journal of the Association of Official Analytical Chemists, 49(3), 554–562.
Silambarasan, S., Singh, R., & Mandal, A.B. (2016). Evaluation of adsorbents to ameliorate the adverse effects of aflatoxin B1 on blood biochemicals, immune response and histopathology of liver in broiler chickens. Indian Journal of Poultry Science, 50(3), 267–271.
Singh, R. (2019a). Aflatoxicosis in buffaloes (A pilot study). Annual Report, ICAR-Central Institute for Research on Buffaloes, Hisar, p. 43–44.
Singh, R. (2019b). Mycotoxins contamination of animal feeds and feed ingredients available in Haryana. Livestock Research International, 7(4), 250–254.
Singh, R. (2019c). Efficacy of choline in ameliorating aflatoxicosis in broiler chickens. International Journal of Current Microbiology and Applied Sciences, 8(2), 2356–2365.
Singh, R. (2020). Impact of certain mycotoxins in dairy animals. Livestock Technology, 9(11), 16–20.
Singh, R., Mandal, A.B., & Shrivastava, A.K. (2013). Amelioration of aflatoxicosis in coloured broiler chickens by dietary butylated hydroxytoluene. Animal Nutrition and Feed Technology, 13, 235–242.
Xiong, J.L., Wang, Y.M., Nennich, T.D., Li, Y., & Liu, J.X. (2023). Transfer of dietary aflatoxin B1 to milk aflatoxin M1 and effect of adsorbent inclusion in dairy cows’ diets. Journal of Dairy Science, 106(2), 1447–1456.
Yiannikouris, A., & Jouany, J.P. (2002). Mycotoxins in feeds and their fate in animals: A review. Animal Research, 51, 81–99.
Yu, A., Wang, H., Cheng, Q., Rajput, S.A., & Qi, D. (2024). The effects of aflatoxin B1 on liver cholestasis and its nutritional regulation in ducks. Toxins, 16(6), 239.
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