Improved In Vitro Maturation of Goat Oocytes using Cysteamine and Epidermal Growth Factor-Enriched Media
DOI:
https://doi.org/10.48165/ijvsbt.21.6.18Keywords:
Cysteamine, Epidermal growth factor, In vitro maturation, GoatAbstract
The current study was performed to see the effect of cysteamine and epidermal growth factor (EGF) on in vitro maturation of goat oocytes (n=8516). The four maturation media used in the study were: Control (TCM-199 + FSH 1 µg/mL + 20 IU/mL hCG + 1 μg/mL Estradiol + Gentamicin 50 μg/mL + 10% EGS); Group I (Control + 100 µM/mL Cysteamine); Group II (Control + 20 ng/mL EGF) and Group III (Control + 100 µM/mL Cysteamine and 20 ng/mL EGF), involving 1591, 1252, 1242, and 1214 oocytes, respectively, for 50, 33, 31, and 30 trials. Later the oocytes were incubated in CO2 incubator for 27 h at 38.5°C, 5% CO₂, and 90-95% relative humidity. The in vitro maturation of goat oocytes, assessed based on cumulus expansion (77.02±2.24 to 77.56±1.57 vs 52.63±1.84%) and first polar body extrusion (55.01±3.00 to 64.11 ±1.93 vs 31.64±2.68%) was significantly higher (p≤0.05) in Group I, II and III compared to Control group. In summary, the addition of cysteamine and epidermal growth factor alone or in combination in the maturation media had a beneficial effect on maturation rate in goat oocytes.
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