Association of Candidate Gene Polymorphisms with Tick Resistance in Kankrej and Holstein-Friesian Crossbred Cattle
DOI:
https://doi.org/10.48165/ijvsbt.21.6.04Keywords:
Candidate gene, Kankrej, PCR-RFLP, Tick resistanceAbstract
This study investigated polymorphisms in four candidate genes (CD14, SPP1, CD209, and EFR3A) previously reported to be associated with tick resistance or immune mechanisms against tick and tick-borne diseases (TTBDs). PCR-RFLP was used to genotype Kankrej and Holstein Friesian crossbred (HFCB) cattle, and associations between gene variants and tick infestation status were evaluated. CD14 and CD209 genes showed polymorphisms in crossbreds, while Kankrej cattle were monomorphic for these loci. The SPP1 and EFR3A genes were monomorphic in both breeds. Although CD14 polymorphisms were not significantly associated with tick resistance, animals with the BB genotype had reduced odds of infestation compared to AB. A significant association was observed for CD209, where the AA genotype conferred a 12.89 fold higher susceptibility to ticks than AB. These findings highlight the potential of CD209 as a genetic marker for breeding programs aimed at enhancing tick resistance, while also emphasizing the breed-specific distribution of polymorphisms in candidate immune genes.
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