Evaluation of Complementary Effects of Exogenous Fibrolytic Enzymes and Fumaric Acid on Methane Emission and Ruminal Fermentation by In Vitro Method
DOI:
https://doi.org/10.48165/ijvsbt.21.6.26Keywords:
Cellulase, Exogenous fibrolytic enzymes, Fumaric Acid, In vitro, Methane, Sodium sulfate, Rumen fermentation, XylanaseAbstract
The objective of the present study was to investigate the hypothesis that exogenous fibrolytic enzymes (EFE, Cellulase with activity >4500-5000 µM glucose/g/min and Xylanase with activity >7500-8000 µM xylose/g/min, mixed in 50:50 w/w), in combination with fumaric acid (FA) and sodium sulfate (SS) may provide complementary effects to mitigate methane emission and enhance in vitro fermentation parameters of a sorghum stover based substrate (60% roughage and 40% concentrate). Three levels of EFE (0, 0.75 and 1.5 g/kg DM) (E0, E1 and E2) with two levels of FA (F0 and F1) @ 0 and 8 mM (or 0 and 2 % of DM) and SS (S0 and S1) @ 0 and 1 g/kg DM, were tested in 3x2x2 factorial design using in vitro gas production technique. E1 level reduced the methane production and improved TDDM (%) significantly while the combination of E1 level with F1 reduced methane % along with enhanced TDDM and TDOM (%). Fumarate alone was not efficient to decrease methane levels and increase digestibility but its combination with E1 level showed the desired effects. Other levels (S1 with E1 and S1F1 with E1) reduced methane but TDDM (%) and TDOM (%) were reduced. Overall, the in vitro results of fumaric acid in combination with EFE 0.75 g/kg DM can provide a possible strategy for mitigation of enteric methane emission to be proved by in vivo experiments.
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Almaraz, I., González, S. S., Pinos-Rodríguez, J. M., & Miranda, L. A. (2010). Effects of exogenous fibrolytic enzymes on in sacco and in vitro degradation of diets and on growth performance of lambs. Italian Journal of Animal Science, 9(1), e2.
Alvarez, G., Pinos-Rodriguez, J. M., Herrera, J. G., Garcia, J. C., Gonzalez, S. S., & Barcena, R. (2009). Effects of exogenous fibrolytic enzymes on ruminal digestibility in steers fed high fiber rations. Livestock Science, 121(2–3), 150–154.
AOAC—Association of Official Analytical Chemists. (2006). Official Methods of Analysis (18th ed.). AOAC International.
Beauchemin, K. A., Kreuzer, M., O’Mara, F., & McAllister, T. A. (2008). Nutritional management for enteric methane abatement: A review. Australian Journal of Experimental Agriculture, 48(2), 21–27.
Blümmel, M., Givens, D. I., & Moss, A. R. (2005). Comparison of methane produced by straw-fed sheep in open-circuit respiration with methane predicted by fermentation characteristics measured by an in vitro gas procedure. Animal Feed Science and Technology, 123, 379–390.
Blümmel, M., Makkar, H. P. S., & Becker, K. (1997). In vitro gas production: A technique revisited. Journal of Animal Physiology and Animal Nutrition, 77(1–5), 24–34.
Blümmel, M., Mgomezulu, R., Chen, X. B., Makkar, H. P. S., Becker, K., & Ørskov, E. R. (1999). The modification of an in vitro gas production test to detect roughage-related differences in in vivo microbial protein synthesis as estimated by the excretion of purine derivatives. The Journal of Agricultural Science, 133(3), 335–340.
Bowman, G. R., Beauchemin, K. A., & Shelford, J. A. (2002). The proportion of the diet to which fibrolytic enzymes are added affects nutrient digestion by lactating dairy cows. Journal of Dairy Science, 85(12), 3420–3429.
Carro, M. D., Ranilla, M. J., & Tejido, M. L. (2005). Using an in vitro gas production technique to examine feed additives: Effects of correcting values for different blanks. Animal Feed Science and Technology, 123, 173–184.
Chung, Y. H., Zhou, M., Holtshausen, L., Alexander, T. W., McAllister, T. A., Guan, L. L., & Beauchemin, K. A. (2012). A fibrolytic enzyme additive for lactating Holstein cow diets: Ruminal fermentation, rumen microbial populations, and enteric methane emissions. Journal of Dairy Science, 95(3), 1419–1427.
Colombatto, D., Hervás, G., Yang, W. Z., & Beauchemin, K. A. (2003). Effects of enzyme supplementation of a total mixed ration on microbial fermentation in continuous culture, maintained at high and low pH. Journal of Animal Science, 81(10), 2617–2627.
Colombatto, D., Mould, F. L., Bhat, M. K., & Owen, E. (2007). Influence of exogenous fibrolytic enzyme level and incubation pH on the in vitro ruminal fermentation of alfalfa stems. Animal Feed Science and Technology, 137(1–2), 150–162.
Krueger, N. A., & Adesogan, A. T. (2008). Effects of different mixtures of fibrolytic enzymes on digestion and fermentation of Bahia grass hay. Animal Feed Science and Technology, 145(1–4), 84–94.
Mao, S., Huo, W., & Zhu, W. (2013). Use of pyrosequencing to characterize the microbiota in the ileum of goats fed with increasing proportion of dietary grain. Current Microbiology, 67(3), 341–350.
McAllister, T. A., Cheng, K. J., Okine, E. K., & Mathison, G. W. (1996). Dietary, environmental and microbiological aspects of methane production in ruminants. Canadian Journal of Animal Science, 76(2), 231–243.
Menke, K. H., & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7–55.
Shojaeian, K., & Thakur, S. S. (2007). Effect of exogenous fibrolytic enzymes supplementation to substrates containing different roughage: Concentrate ratios on in vitro rumen fermentation, DM and NDF degradability. Indian Journal of Dairy Science, 60(2), 94–101.
Soltan, Y. A., Abdalla, A. L., Silva, L. R., Natel, A. S., Morsy, A. S., & Louvandini, H. (2013). Response of different tropical pasture grass species to treatments with fibrolytic enzymes in terms of in vitro ruminal nutrient degradation and methanogenesis. Animal Nutrition and Feed Technology, 13(3), 551–568.
Stewart, C. S., Flint, H. J., & Bryant, M. P. (1997). The rumen bacteria. In P. N. Hobson & C. S. Stewart (Eds.), The Rumen Microbial Ecosystem (pp. 10–72). Springer.
Takahashi, J., Johchi, N., & Fujita, H. (1989). Inhibitory effects of sulphur compounds, copper and tungsten on nitrate reduction by mixed rumen micro-organisms. British Journal of Nutrition, 61(3), 741–748.
Tang, S. X., Tayo, G. O., Tan, Z. L., Sun, Z. H., Shen, L. X., Zhou, C. S., & Shen, S. B. (2008). Effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristics of low-quality cereal straws. Journal of Animal Science, 86(5), 1164–1172.
Tavendale, M. H., Meagher, L. P., Pacheco, D., Walker, N., Attwood, G. T., & Sivakumaran, S. (2005). Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology, 123, 403–419.
Thakur, S. S., Tomar, S. K., & Sirohi, S. K. (2008). In vitro DM and cell wall degradability of total mixed rations influenced by exogenous fibrolytic enzymes supplementation. Indian Journal of Animal Nutrition, 25(3), 219–223.
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583–3597.
Wallace, R. J., Wallace, S. J. A., McKain, N., Nsereko, V. L., & Hartnell, G. F. (2000). Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro. Journal of Animal Science, 78(7), 1905–1916.
Wang, L., & Xue, B. (2016). Effects of cellulase supplementation on nutrient digestibility, energy utilization and methane emission by Boer crossbred goats. Asian-Australasian Journal of Animal Sciences, 29(2), 204–210.
Wood, T. A., Wallace, R. J., Rowe, A., Price, J., Yáñez-Ruiz, D. R., Murray, P., & Newbold, C. J. (2009). Encapsulated fumaric acid as a feed ingredient to decrease ruminal methane emissions. Animal Feed Science and Technology, 152(1–2), 62–71.
Yang, W. Z., Beauchemin, K. A., & Rode, L. M. (2000). A comparison of methods of adding fibrolytic enzymes to lactating cow diets. Journal of Dairy Science, 83(11), 2512–2520.
Zhao, L., Peng, Y., Wang, J., & Liu, J. (2015). Effects of exogenous fibrolytic enzyme on in vitro ruminal fiber digestion and methane production of corn stover and corn stover-based mixed diets. Life Science Journal, 12(2s), 1–9.
Zhou, M., Chung, Y. H., Beauchemin, K. A., Holtshausen, L., Oba, M., McAllister, T. A., & Guan, L. L. (2011). Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive. Journal of Applied Microbiology, 111(5), 1148–1158.
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