Induction of Estrus in Postpartum Anestrus Murrah Buffaloes using Chelated Minerals and Ovsynch Protocol
DOI:
https://doi.org/10.48165/ijvsbt.21.6.14Keywords:
Buffalo, Chelated mineral mixture, Estrus induction, Mineral profile, Ovsynch, Postpartum anestrusAbstract
A research trial aimed to assess the effect of chelated mineral mixture supplementation and Ovsynch protocol on induction of estrus was conducted on 120 days postpartum true anestrus Murrah buffaloes. A total of 18 true postpartum acyclic buffaloes selected were divided into three groups each of 6 animals. Group T1 was treated with chelated mineral mixture Agrimin Forte (50 g/day) and Garbhov bolus (1 bolus/day) for 15 days. In Group T2, chelated mineral mixture as in T1 plus Ovsynch protocol comprising intramuscular injections of Buserelin acetate (10 µg), Cloprostenol (500 µg), and Buserelin acetate (10 µg) on days 0, 7 and 9, respectively, were given, and treated buffaloes were inseminated after detected heat. The control group (C) buffaloes received no treatment. Blood glucose, and serum macro-micro minerals profile was compared pre- and post-treatment between the groups. Estrus was induced in 66.67% and 83.33% of postpartum anestrus buffaloes, respectively, in T1 and T2 groups with intermediate to intense estrus intensities, as compared to the untreated control group (33.33%). After supplementation of the mineral mixture (T1), concentrations of blood glucose, calcium, magnesium, copper, iron, and zinc increased significantly (p≤0.05); however, inorganic phosphorus, cobalt, and selenium levels did not vary significantly post-treatment. In group T2, a significant increase was observed in the mean concentrations of blood glucose, copper, and cobalt. From the present study, it can be concluded that supplementation of mineral mixture and application of hormonal synchronization protocol successfully induced estrus in postpartum acyclic Murrah buffaloes and can be adopted as a potential tool to alleviate the inherited problems of poorly exhibited estrus.
Downloads
References
Bailey, J.D., Ansotegui, R.P., Paterson, J.A., Swenson, C.K., & Johnson, A.B. (2001). Effects of supplementing combinations of inorganic and complexed copper on performance and liver mineral status of beef heifers consuming antagonists. Journal of Animal Science, 79, 2926–2934.
Biswas, J., Ray, S.K., Chattarajee, S., & Mukherjee, K.P. (2005). Effect of feeding extra vitamin-minerals with ration on serum biochemical profile and reproduction in anestrus Murrah buffaloes during low-breeding season. Journal of Ecology and Environment, 23, 577–579.
El-Wishy, A.B. (2007). The postpartum buffalo. II. Acyclicity and anestrus. Animal Reproduction Science, 97(3–4), 216–236.
Georgievskii, V.I. (1982). Mineral composition of bodies and tissue of animals. In Mineral Nutrition of Animals (Vol. 1, pp. 66–67). Butterworths, London, England.
Joshi, P.M., Patel, D.C., Patel, P.D., & Sarvaiya, N.P. (2020). Effect of chelated mineral mixture on blood profile and fertility in anoestrus buffaloes of tribal areas of Dahod district in Gujarat, India. Indian Journal of Veterinary Science and Biotechnology, 15(3), 61–65.
Judson, G.J., & McFarlane, J.D. (1998). Minerals disorders in grazing livestock and the usefulness of soil and plant analysis in the assessment of these disorders. Australian Journal of Experimental Agriculture, 38, 707–723.
Karena, M., & Darwish, A. (2010). Efficacy of Ovsynch protocol in cyclic and acyclic Egyptian buffaloes in summer. Animal Reproduction Science, 119, 17–23.
Kumar, S., Balhara, A.K., Kumar, R., Kumar, N., Buragohain, L., Baro, D., Sharma, R.K., Phulia, S.K., & Singh, I. (2015). Hemato biochemical and hormonal profiles in post-partum water buffaloes (Bubalus bubalis). Veterinary World, 8, 512–517.
Lall, D., Sikka, P., Khanna, S., & Chauhan, T.R. (2004). Effect of high plan of nutrition and mineral supplementation on blood mineral profile, thyroid hormones and fertility status of anoestrus buffaloes. Animal Nutrition and Feed Technology, 4, 71–76.
Marai, I.F.M., & Haeeb, A.A.M. (2010). Buffalo’s biological functions as affected by heat stress – A review. Journal of Livestock Science, 127(2–3), 89–109.
Maurya, S.K., Sagar, R., Panigrahi, P.N., & Saxena, A. (2021). Studies on blood mineral and hormonal profile in post-partum anestrus buffalo. Journal of Entomology and Zoology Studies, 9(1), 1913–1917.
Mujawar, A.S., Razzaque, W.A.A., Ramteke, S.S., Patil, A.D., Ali, S.S., Bhikane, A.U., & Mogal, I.R. (2019). Estrus induction and fertility response in postpartum anoestrus Marathwadi buffaloes using hormonal protocol along with vitamin E and Selenium. International Journal of Livestock Research, 9(3), 289–296.
Nakrani, B.B., Panchal, M.T., Dhami, A.J., Hadiya, K.K., Patel, J.A., Gosai, R.K., & Shah, R.G. (2014). Influence of controlled breeding techniques on estrus induction response, conception rate and plasma progesterone profile in anoestrus buffaloes. Global Journal of Medical Research, 14(3), 1–6.
Orskov, E.R. (2007). Some physical, physiological and biochemical adaptations of ruminant livestock including buffaloes to different feeds and climates. Italian Journal of Animal Science, 6(sup2), 223–226.
Pande, N., Agrawal, R., Shrivastava, O.P., & Swamy, M. (2016). Alterations in haemato-metabolic status and body condition score of buffaloes during the transition period. Journal of Livestock Science, 7, 122–125.
Perera, B.M.A.O. (2011). Reproductive cycles of buffalo. Animal Reproduction Science, 124, 1094–1099.
Rao, S.V., & Rao, A.R. (1981). Oestrus behaviour and ovarian activity of crossbred heifers. Indian Veterinary Journal, 58, 881–884.
Ravikumar, K., Asokan, S.A., & Veerapandian, C. (2007). Inclusion of CIDR in Ovsynch protocol to improve fertility in postpartum anestrus buffaloes. Tamilnadu Journal of Veterinary and Animal Science, 3, 24–28.
Richards, M.W., Wettemann, R.P., & Schoenemann, H.M. (1989). Nutritional anestrus in beef cows: concentrations of glucose and nonesterified fatty acids in plasma and insulin in serum. Journal of Animal Science, 67(9), 2354–2362.
Savalia, K.K., Dhami, A.J., Patel, K.R., & Hadiya, K.K. (2013). Influence of controlled breeding techniques on fertility and plasma macro minerals profile in conceiving and non-conceiving anoestrus and repeat breeding buffaloes. Indian Journal of Veterinary Sciences and Biotechnology, 9(2), 28–35.
Selvaraju, S., Reddy, I.J., Gowda, N.K.S., Prasad, C.S., Ananthram, K., & Sampath, K.T. (2009). Effect of supplementation of area specific mineral mixture in improving reproductive efficiency in crossbred dairy cattle—a field study. Indian Journal of Animal Sciences, 79(6), 599–601.
Setia, M.S., Duggal, R.S., Singh, R., & Singh, R. (1994). Distribution of trace elements in whole blood plasma during late pregnancy and different stages of lactation in buffaloes and cows. Buffalo Journal, 3, 213–220.
Singh, J., Ghuman, S.P.S., Honparkhe, M., & Singh, N. (2009). Investigations on dominant follicle development, estrus response, ovulation time and fertility in PRID-treated anestrus buffalo heifers. Indian Journal of Animal Sciences, 79(8), 773.
Singh, S., Chhabra, S., Singh, C., Randhawa, S.S., & Gupta, D.K. (2016). Effect of area specific mineral mixture feeding on milk yield and composition of dairy animals of central zone of Punjab. International Journal of Livestock Research, 6(3), 62–65.
Snedecor, G.W., & Cochran, W.G. (1994). Statistical Methods (8th ed.). Oxford and IBH Publishing Co., New Delhi, India, pp. 445–446.
Thorat, T., Patil, A.D., Kumbhar, U.B., & Ghoke, S.S. (2012). Improving fertility in postpartum Marathwadi anoestrus buffaloes using ovsynch and selectsynch protocols. Indian Journal of Animal Reproduction, 33(2), 58–59.
Virmani, M., Malik, R.K., & Singh, P. (2018). Effect of gonadotropin releasing hormone (GnRH) preparations on induction of estrus and fertility in buffaloes under field conditions in Haryana. Buffalo Bulletin, 35(1), 93–100.
Yildiz, A., Balikci, E., & Gurdogan, F. (2005). Serum mineral levels at pregnancy and postpartum in single and twin pregnant sheep. Biological Trace Element Research, 107, 247–253.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Indian Journal of Veterinary Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
