Resistance to Benzimidazoles in Haemonchus, Trichostrongylus and Strongyloides species in Sheep Farms of Three Agro-Climatic Zones of Haryana, India
DOI:
https://doi.org/10.48165/ijvsbt.21.6.24Keywords:
Anthelminthic resistance, Benzimidazoles, Egg hatch assay (EHA), Faecal egg count reduction test (FECRT), Haryana, SheepAbstract
The regular and frequent use of similar anthelmintics has led to the development and prevalence of anthelmintic resistance which hinders the control of gastrointestinal nematodes (GINs) in sheep. The present research work was planned with the aim of studying the status of benzimidazole group of drug to GINs in sheep farms (one organized and 3 unorganized) of arid (Zone-I, Hisar), semi-arid (Zone-II, Kaithal) and dry sub-humid (Zone-III, Ambala) Zones of Haryana, India using in vivo faecal egg count reduction test (FECRT) and in vitro egg hatch assay (EHA). The FECRT resulted moderate resistance (60.60-75.26%) in all the selected sheep farms against fenbendazole @ 5 mg/kg b.wt. orally. The faecal culture revealed the predominance of H. contortus infective larvae with few Trichostrongylus spp. and Strongyloides spp. However, EHA revealed resistance in two farms (0.108-0.110 µg TBZ/mL) and susceptibility (0.087-0.092 µg TBZ/mL) of other two farms to thiabendazole, i.e., there ED50 values were below 0.1 µg TBZ/mL. This seems to be the first study of anthelmintic resistance against GINs in sheep farms from three agro-climatic zones of Haryana. This basic knowledge on resistance of benzimidazoles group of drug will help in formulating a strategy to control gastrointestinal nematodes in sheep of Haryana.
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Ancheta, P.B., Dumilon, R.A., Venturina, V.M., Cerbito, W.A., Dobson, R.J., LeJambre, L.F., & Gray, G.D. (2004). Efficacy of benzimidazole anthelmintics in goats and sheep in the Philippines using a larval development assay. Veterinary Parasitology, 120(1-2), 107-121.
Arsenopoulos, K., Minoudi, S., Symeonidou, I., Triantafyllidis, A., Katsafadou, A.I., Lianou, D.T., & Papadopoulos, E. (2020). Frequency of resistance to benzimidazoles of Haemonchus contortus helminths from dairy sheep, goats, cattle and buffaloes in Greece. Pathogens, 9(5), 347.
Coles, G.C., Jackson, F., Pomroy, W.E., Prichard, R.K., von Samson-Himmelstjerna, G., Silvestre, A., Taylor, M.A., & Vercruysse, J. (2006). The detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 136, 167-185.
DAHD - Department of Animal Husbandry and Dairying, Government of Haryana. (2020). 20th Livestock Census. Retrieved from http://pashudhanharyana.gov.in/livestock-census-0
Dey, A.R., Begum, N., Alim, M.A., & Alam, M.Z. (2020). Multiple anthelmintic resistance in gastrointestinal nematodes of small ruminants in Bangladesh. Parasitology International, 77, 102-105.
Dixit, A.K., Das, G., Dixit, P., Singh, A.P., Kumbhakar, N.K., Sankar, M., & Sharma, R.L. (2017). An assessment of benzimidazole resistance against caprine nematodes in Central India. Tropical Animal Health and Production, 49(7), 1471-1478.
Drudge, J.H., Szanto, J., Wyant, Z.N., & Elam, G. (1964). Field studies on parasitic control in sheep: comparison of thiabendazole, ruelene and phenothiazine. American Journal of Veterinary Research, 25, 1512-1518.
Falzon, L.C., Menzies, P.I., Vanleeuwen, J., Jones-Bitton, A., Shakya, K.P., Avula, J., Jansen, J.T., & Peregrine, A.S. (2013). A survey of farm management practices and their associations with anthelmintic resistance in sheep flocks in Ontario, Canada. Small Ruminant Research, 114(1), 41-45.
Hunt, K.R., & Taylor, M.A. (1989). Use of the egg hatch assay on sheep faecal samples for the detection of benzimidazole resistant worms. Veterinary Record, 125, 153-154.
Kotze, A.C., & Prichard, R.K. (2016). Anthelmintic resistance in Haemonchus contortus: history, mechanisms and diagnosis. Advances in Parasitology, 93, 397-428.
Kumar, S., & Singh, S. (2016). Detection of multiple anthelmintic resistance against gastrointestinal nematodes in sheep on Central Sheep Breeding Farm, Hisar. Haryana Veterinarian, 55(2), 210-213.
Maharshi, A.K., Swarnkar, C.P., Singh, D., Manohar, G.S., & Ayub, M. (2011). Status of anthelmintic resistance in gastrointestinal nematodes of sheep in Rajasthan. Indian Journal of Animal Sciences, 81, 105-109.
Maingi, N., Bjorn, H., & Dangolla, A. (1998). The relationship between faecal egg count reduction test and the lethal dose 50% in the egg hatch assay and larval development assay. Veterinary Parasitology, 77, 133-145.
Martin, R.J. (1997). Modes of action of anthelmintic drugs. Veterinary Journal, 154(1), 11-34.
McKellar, Q.A., & Jackson, F. (2004). Veterinary anthelmintics: old and new. Trends in Parasitology, 20(10), 456-461.
Ploeger, H.W., & Everts, R.R. (2018). Alarming levels of anthelmintic resistance against gastrointestinal nematodes in sheep in the Netherlands. Veterinary Parasitology, 262, 11-15.
Rialch, A., Vatsya, S., & Kumar, R.R. (2013). Detection of benzimidazole resistance in gastrointestinal nematodes of sheep and goats of sub-Himalayan region of northern India using different tests. Veterinary Parasitology, 198(3-4), 312-318.
Santiago-Figueroa, I., Lara-Bueno, A., Gonzalez-Garduno, R., Lopez-Arellano, M.E., de la Rosa-Arana, J.L., & de Jesus Maldonado-Siman, E. (2019). Anthelmintic resistance in hair sheep farms in a sub-humid tropical climate, in the Huasteca Potosina, Mexico. Veterinary Parasitology, 17, 100-292.
Sharma, R., Singh, S., & Vohra, S. (2015). Detection of anthelmintic resistance in gastrointestinal nematodes of sheep on Government Sheep Breeding Farm, Hisar. Haryana Veterinarian, 54(2), 47-149.
Vadlejch, J., Kopecky, O., Kudrnacova, M., Cadkova, Z., Jankovska, I., & Langrova, I. (2014). The effect of risk factors of sheep flock management practices on the development of anthelmintic resistance in the Czech Republic. Small Ruminant Research, 117, 183-190.
Van Wyk, J.A. (1990). Occurrence and dissemination of anthelmintic resistance in South Africa and management of resistant worm strains. In Proceedings of the Round Table Conference of ICOPA VII on Resistance of Parasites to Antiparasitic Drugs (pp. 103-114).
Van Wyk, J.A., & Mayhew, E. (2013). Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide. Onderstepoort Journal of Veterinary Research, 80(1), 539-553.
Varady, M., Cudekova, P., & Corba, J. (2007). In vitro detection of benzimidazole resistance in Haemonchus contortus: egg hatch test versus larval development test. Veterinary Parasitology, 149(1-2), 104-110.
von Samson-Himmelstjerna, G., Coles, G.C., Jackson, F., et al. (2009). Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes. Parasitology Research, 105(3), 825-834.
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