The Usage of Progesterone Hormone and Ultrasonography for Early Pregnancy Detection in Local Iraqi Ewes
DOI:
https://doi.org/10.48165/ijar.2025.46.03.21Keywords:
Ewes, Pregnancy, Ultrasound, ProgesteroneAbstract
The study was designed to evaluate and compare the reliability of several diagnostic methods for early pregnancy detec tion. Fifteen multiparous, non-pregnant local Iraqi ewes aged 2–4 years, along with four breeding rams, were used in this experiment. All animals were housed at the College of Veterinary Medicine, University of Fallujah, from October 2024 to March 2025. Estrus synchronization in the experimental ewes was performed using Medroxyprogesterone Acetate (MPA) intravaginal sponges combined with 500 IU of eCG, followed by natural mating with the four rams. Mating occurred at the onset of estrus and this time point was considered day 0. Whole blood samples were collected from the 15 ewes on day 0, and additional samples were obtained on days 14, 21, and 30 post-insemination (PI) for serum pro gesterone (P4) analysis. Early pregnancy was assessed using transrectal ultrasonography (TRU) with a 7.5 MHz probe. Serum samples were also used to measure P4 concentrations. Based on the findings, the detection Accuracy (Acc) of ULR was low in early pregnancy (days 14-21 PI) and improved along with pregnancy progression (30 PI). Progesterone levels were significantly raised in pregnant ewes compared to non-pregnant and control throughout the period. In days 14-21 PI, the P4 test method was significantly more effective than ULR in predicting pregnancy. By day 30 PI, the reli ability of all methods improved, the P4 assay demonstrated approximately similar efficiency to ULR. In conclusion, the P4 assay is a more reliable method for detecting pregnancy during the early stages (days 14–21 PI). From day 30 onward, ultrasonography becomes a superior approach due to its higher reliability, cost-effectiveness, and ability to determine both pregnancy status and embryo/fetal number.
References
Abas, H. K., EA, A., Al-Hamedawi, T. M., & Ajeel, H. M. (2022). Pregnancy rate in synchronized Iraqi Awassi ewes inseminated artificially and naturally. Indian Journal of Forensic Medicine & Toxicology, 16(1), 1–6. https://doi.org/10.37506/ijfmt.v16i1.17808
Aboud, Q. M. (2024). Individual variation in semen attributes and number of straws produced by Holstein bulls born in Iraq during different ages and months. Egyptian Journal of Veterinary Sciences, 55(7), 1857–1867. https://doi.org/10.21608/ejvs.2024.263952.1790
Aldeen, E., Albayati, O. A., & Ismaeel, M. A. (2022). Study of some biochemical and vitamins changes in the occurrence of repeated fertilization failure in sheep. Annals of the Romanian Society for Cell Biology, 26(01), 3262–3269. http://annalsofrscb.ro/index.php/journal/article/view/11179
Alexander, B., Coppola, G., Mastromonaco, G. F., John, E., Reyes, E. R., Betts, D. H., & King, W. A. (2008). Early pregnancy diagnosis by serum progesterone and ultrasound in sheep carrying somatic cell nuclear transfer-derived pregnancies. Reproduction in Domestic Animals, 43(2), 207–211. https://doi.org/10.1111/j.1439-0531.2007.00878.x
Ali, A., & Hayder, M. (2007). Ultrasonographic assessment of embryonic, fetal, and placental development in Ossimi sheep. Small Ruminant Research, 73(1–3), 277–282. https://doi.org/10.1016/j.smallrumres.2007.01.009
Al-Sariy, S. M., Al-Yasiri, E. A., & Al-Hamedawi, T. M. (2020). Limitation of gestational age in local Iraqi ewes and does by using number and measurement of placentomes diameter in abattoirs. Biochemical and Cellular Archives, 20(1), 1089–1191. https://doi.org/10.35124/bca.2020.20.1.1089
Al-Thuwaini, T. M., & Al-Hadi, A. B. (2022). Association of lamb sex with body measurements in single and twin Awassi ewes. Advances in Animal and Veterinary Sciences, 10(8), 1849–1853. https://dx.doi.org/10.17582/journal.aavs/2022/10.8.1849.1853
Alwan, A. F., Amin, F. A., & Ibrahim, N. S. (2010). Blood progesterone and estrogen hormones level during pregnancy and after birth in Iraqi sheep and goat. Basrah Journal of Veterinary Research, 9(2), 153–157. https://doi.org/10.33762/bvetr.2010.55059
Ataman, M. B., Aköz, M., Saribay, M. K., Erdem, H., & Bucak, M. N. (2013). Prevention of embryonic death using different hormonal treatments in ewes. Turkish Journal of Veterinary and Animal Sciences, 37(1), 6–8. https://doi.org/10.3906/vet-1102-795
Balaro, M. F., Cosentino, I. O., Ribeiro, A. C., & Brandão, F. Z. (2022). Ultrasound diagnosis in small ruminants: Occurrence and description of genital pathologies. Veterinary Sciences, 9(11), 599. https://doi.org/10.3390/vetsci9110599
Barbagianni, M. S., Ioannidi, K. I., Vasileiou, N. G., Mavrogianni, V. S., Orfanou, D. C., Fthenakis, G. C., & Valasi, I. (2017). Ultrasonographic examination of pregnant ewes: From early diagnosis of pregnancy to early prediction of dystocia. Small Ruminant Research, 152, 41–55. https://doi.org/10.1016/j.smallrumres.2016.12.008
Boscos, C. M., Samartzi, F. C., Lymberopoulos, A. G., Stefanakis, A., & Belibasaki, S. (2003). Assessment of progesterone concentration using enzyme immunoassay for early pregnancy diagnosis in sheep and goats. Reproduction in Domestic Animals, 38(3), 170–174. https://doi.org/10.1046/j.1439-0531.2003.00407
DeNicolo, G., Parkinson, T. J., Kenyon, P. R., Morel, P. C., & Morris, S. T. (2009). Plasma progesterone concentrations during early pregnancy in spring- and autumn-bred ewes. Animal Reproduction Science, 111(2–4), 279–288. https://doi.org/10.1016/j.anireprosci.2008.02.009
Dhahir, N. N., Ismaeel, M. A., & Aldoori, Z. T. (2022). Effect of adding carrots as feed supplementation on reproductive performance in Awassi ewes. Iraqi Journal of Veterinary Sciences, 36(2), 413–417. https://doi.org/10.33899/ijvs.2022.132924.2159
Edwards, S. J., Smaill, B., O’Connell, A. R., Johnstone, P. D., Stevens, D. R., Quirke, L. D., Farquhar, P. A., & Juengel, J. L. (2016). Reduced ovulation rate, failure to be mated and fertilization failure/embryo loss are the underlying causes of poor reproductive performance in juvenile ewes. Animal Reproduction Science, 167, 125–132. https://doi.org/10.1016/j.anireprosci.2016.02.008
Ganaie, B. A., Khan, M. Z., Islam, R., Makhdoomi, D. M., Qureshi, S., & Wani, G. M. (2009). Evaluation of different techniques for pregnancy diagnosis in sheep. Small Ruminant Research, 85(2–3), 135–141. https://doi.org/10.1016/j.smallrumres.2009.07.004
Garcia, A., Neary, M. K., Kelly, G. R., & Pierson, R. A. (1993). Accuracy of ultrasonography in early pregnancy diagnosis in the ewe. Theriogenology, 39(4), 847–861. https://doi.org/10.1016/0093-691X(93)90041-8
Gür, S., Türk, G., Demirci, E., Yüce, A., Sönmez, M., Özer, Ş., & Aksu, E. H. (2011). Effect of pregnancy and foetal number on diameter of corpus luteum, maternal progesterone concentration and oxidant/antioxidant balance in ewes. Reproduction in Domestic Animals, 46(2), 289–295. https://doi.org/10.1111/j.1439-0531.2010.01653.x
Ismaeel, M. A., Saed, O. S., & Dhahir, N. N. (2023). Effect of different gestation periods on serum estrogen, progesterone and some biochemical parameters in Awassi ewes. Iraqi Journal of Agricultural Sciences, 54(3), 884–889. https://doi.org/10.36103/ijas.v54i3.1870
Jainudeen, M. R., & Hafez, E. S. E. (2013). Pregnancy diagnosis. In E. S. E. Hafez & B. Hafez (Eds.), Reproduction in farm animals (7th ed., pp. 134–145). Wiley-Blackwell. https://doi.org/10.1002/9781119265306.ch20
Kaçar, C., Kaya, S., & Demir, M. C. (2022). Effects of antioxidant compounds on fertility in small ruminants. In The role of exogenous antioxidants in enhancing reproductive function and performance (pp. 71–88). IntechOpen. https://doi.org/10.5772/intechopen.100312
Kadhem, A. F., & Al-Thuwaini, T. M. (2022). Influence of litter size on the hematologic profile of Awassi ewes during gestation and lactation. Veterinary Integrative Sciences, 20(3), 625–633. https://doi.org/10.12982/VIS.2022.047
Karen, A., Beckers, J. F., Sulon, J., de Sousa, N. M., Szabados, K., Reczigel, J., & Szenci, O. (2003). Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology, 59(9), 1941–1948. https://doi.org/10.1016/S0093-691X(02)01289-X
Karen, A., El Amiri, B., Beckers, J. F., Sulon, J., Taverne, M. A., & Szenci, O. (2006). Comparison of accuracy of transabdominal ultrasonography, progesterone and pregnancy-associated glycoproteins tests for discrimination between single and multiple pregnancy in sheep. Theriogenology, 66(2), 314–322. https://doi.org/10.1016/j.theriogenology.2005.11.010
Karen, A., Szabados, K., Reiczigel, J., Beckers, J. F., & Szenci, O. (2004). Accuracy of transrectal ultrasonography for determination of pregnancy in sheep: Effect of fasting and handling of the animals. Theriogenology, 61(7–8), 1291–1298. https://doi.org/10.1016/j.theriogenology.2003.07.005
Kaya, A., Aksoy, M., & Tekeli, T. (2002). Influence of ejaculation frequency on sperm characteristics, ionic composition and enzymatic activity of seminal plasma in rams. Small Ruminant Research, 44(2), 153–158. https://doi.org/10.1016/S0921-4488(02)00019-4
Khames, M. M., Shareef, S. O., & Abdulealah, I. M. (2023). Clinical and biochemical study of pregnancy toxemia in Iraqi ewes. Archives of Razi Institute, 78(3), 1131–1139. https://doi.org/10.22092/ari.2022.359420.2029
Kose, M., Hitit, M., Kaya, M. S., Kirbas, M., Dursun, S., Alak, I., & Atli, M. O. (2022). Expression pattern of microRNAs in ovine endometrium during the peri-implantation. Theriogenology, 191, 35–46. https://doi.org/10.1016/j.theriogenology.2021.09.012
Kose, M., Kaya, M. S., Aydilek, N., Kucukaslan, I., Bayril, T., Bademkiran, S., Kiyma, Z. E., Ozyurtlu, N., Kayis, S. A., Guzeloglu, A., & Atli, M. O. (2016). Expression profile of interferon tau–stimulated genes in ovine peripheral blood leukocytes during embryonic death. Theriogenology, 85(6), 1161–1166. https://doi.org/10.1016/j.theriogenology.2015.11.017
Kutlu, M., & Dinç, D. A. (2021). The effect of double-dose GnRH injections on reproductive performance parameters following short-term progestagen administration in lactated Awassi ewes during the non-breeding season. Tropical Animal Health and Production, 53(2), 277. https://doi.org/10.1007/s11250-021-02735-x
Mandiki, S. N., Derycke, G., Bister, J. L., & Paquay, R. (1998). Influence of season and age on sexual maturation parameters of Texel, Suffolk and Ile-de-France rams: 1. Testicular size, semen quality and reproductive capacity. Small Ruminant Research, 28(1), 67–79. https://doi.org/10.1016/S0921-4488(97)00093-3
Martemucci, G., & D’Alessandro, A. G. (2011). Synchronization of oestrus and ovulation by short time combined FGA, PGF2α, GnRH, eCG treatments for natural service or AI fixed-time. Animal Reproduction Science, 123(1–2), 32–39. https://doi.org/10.1016/j.anireprosci.2011.01.011
Martinez-Ros, P., Astiz, S., Garcia-Rosello, E., Rios-Abellan, A., & Gonzalez-Bulnes, A. (2018). Effects of short term intravaginal progestagens on the onset and features of estrus, preovulatory LH surge and ovulation in sheep. Animal Reproduction Science, 197, 317–323. https://doi.org/10.1016/j.anireprosci.2018.09.002
MedCalc Software Ltd. (2016). MedCalc Statistical Software (Version 16.4.3) [Computer software]. https://www.medcalc.org
Mendoza, M. R., Montaldo, H. H., Sánchez, J. A., Mendoza, J. H., & Cerón, J. H. (2009). Serum progesterone levels in Pelibuey and Suffolk ewes under thermal stress. Veterinaria México, 40(2), 197–202.
Metodiev, N., Dimov, D., Ralchev, I., & Raicheva, E. (2017). Efficiency of transrectal ultrasonography for pregnancy diagnosis of Ile de France ewes during the first month of gestation. Bulgarian Journal of Veterinary Medicine, 20(3), 276–280. https://doi.org/10.15547/bjvm.944
Mohammed, T. R., Majeed, A. F., & Alawiy, I. K. (2020). Pregnancy diagnosis in local Iraqi ewes: A comparative study. Annals of Tropical Medicine and Public Health, 23(7), 1119–1125. http://doi.org/10.36295/ASRO.2020.23736
Mohsin, M. K., & Hussein, K. A. (2022). Monitoring influence of PGF2α, estrogen and oxytocin on postpartum uterine involution by ultrasonography in ewes. Indian Journal of Ecology, 49(2), 651–654. https://doi.org/10.55362/IJE/2022/3575
Pagamici, C., & Stephan, R. (2022). Pregnancy in slaughtered lambs and sheep—A cross-sectional study in three abattoirs in Switzerland. Animals, 12(10), 1328. https://doi.org/10.3390/ani12101328
Pasciu, V., Nieddu, M., Baralla, E., Porcu, C., Sotgiu, F., & Berlinguer, F. (2022). Measurement of progesterone in sheep using a commercial ELISA kit for human plasma. Journal of Veterinary Diagnostic Investigation, 34(1), 90–93. https://doi.org/10.1177/10406387211040654
Rahman, M. H., Munsi, M. N., & Akther, S. (2020). Comparative study of pregnancy diagnosis in ewes (Ovis aries) using barium chloride and progesterone-kit. Bangladesh Veterinarian, 37(1–2), 36–41. https://doi.org/10.3329/bvet.v37i1-2.59897
Romano, J. E., & Christians, C. J. (2008). Early pregnancy diagnosis by transrectal ultrasonography in ewes. Small Ruminant Research, 77(1), 51–57. https://doi.org/10.1016/j.smallrumres.2008.02.009
Santos, V. J., Rodriguez, M. G., da Silva, P. D., Mariano, R. S., Taira, A. R., de Almeida, V. T., Uscategui, R. A., Nociti, R. P., Teixeira, P. P., Feliciano, M. A., & Vicente, W. R. (2018). B-mode ultrasonography and ecobiometric parameters for assessment of embryonic and fetal development in sheep. Animal Reproduction Science, 197, 193–202. https://doi.org/10.1016/j.anireprosci.2018.08.007
Santos-Jimenez, Z., Martínez-Ros, P., Encinas, T., Morales Cruz, J. L., Guerrero-Gallegos, H. Z., Gonzalez-Avalos, R., Gonzalez-Bulnes, A., & Guillen-Muñoz, J. M. (2022). Ovarian response and fertility after short-term progestagen/eCG treatments are compromised in nulliparous sheep during non-breeding season. Veterinary Sciences, 9(12), 663. https://doi.org/10.3390/vetsci9120663
See, K., Bailer, A. J., Behnke, E. J., Baker, R. S., Clark, K. E., & Russell, P. T. (2007). Predicting pregnancy status and fetal number in time-dated pregnant ewes using serum progesterone and ultrasound. Research Journal of Animal Sciences, 1(2), 65–71.
SPSS Inc. (2019). IBM SPSS Statistics for Windows (Version 26.0) [Computer software]. IBM Corp.
Tasal, I., Dinc, D., Erdem, H., & Semacan, A. (2006). Pregnancy diagnosis in ewes by real-time ultrasound. Indian Veterinary Journal, 83(9), 968–969.
Titi, H. H., Alnimer, M. A., & Abedal-majed, M. A. (2022). Effect of supplemental rumen-protected methionine on reproduction and production of Awassi ewes. Italian Journal of Animal Science, 21(1), 624–633. https://doi.org/10.1080/1828051X.2022.2030647
Yotov, S. (2005). Diagnostics of early pregnancy in Stara Zagora dairy sheep breed. Bulgarian Journal of Veterinary Medicine, 8(1), 41–45.
Yotov, S. (2007). Determination of the number of fetuses in sheep by means of blood progesterone assay and ultrasonography. Bulgarian Journal of Veterinary Medicine, 10(3), 185–193.
Yu, X., Bai, Y., Yang, J., Zhao, X., Zhang, L., & Wang, J. (2022). Comparison of five protocols of estrous synchronization on reproductive performance of Hu sheep. Frontiers in Veterinary Science, 9, 843514. https://doi.org/10.3389/fvets.2022.843514
Zarco, L., Stabenfeldt, G. H., Quirke, J. F., Kindahl, H., & Bradford, G. E. (1988). Release of prostaglandin F-2α and the timing of events associated with luteolysis in ewes with oestrous cycles of different lengths. Journal of Reproduction and Fertility, 83(2), 517–526. https://doi.org/10.1530/jrf.0.0830517
