Potential Use of Mosambi Peel-Based Bioenzymes for the Wound Management in Animals
DOI:
https://doi.org/10.48165/ijar.2025.46.01.10Keywords:
Bioenzymes, Waste management, Biotherapeutics, Mosambi peel, Wound managementAbstract
Wound healing is one of the major issues faced by animal rearers. The common afflictions are horn and foot injuries in animals. The therapeutic treatment involves the usage of costly antibiotics, which not only causes resistance to antibi otics but also alters the gut microflora. The present study was carried out with the objective to produce mosambi-peel based bioenzymes having antioxidant properties and further exploited for animal wound healing. The bioenzymes were characterized with respect to physico-chemical, enzymatic, color and microbiological profile. The presence of various antioxidant compounds including pterostilbene, hesperetin, aloin A, ononin, quercetin 3,7-dirhamnoside, isoorientin 2’-O-rhamnoside, saponarin was confirmed by LC-MS. Further, the bioenzyme solution sprayed on animal wounds stopped bleeding within 2-3 minutes and showed normal healing indicated by granulation tissue without pus pocket formation or any other side effects even in absence of any antibiotic treatment. In addition to this, it also acted as fly repellent for 5-6 h. Thus, the mosambi-peel based bioenzymes could serve as a cost-effective alternative to swap anti biotic therapy for wound healing in animals. Furthermore, the issue of solid waste management will be addressed by valorization of horticultural waste to bioenzymes.References
Adetunji A.I., Oberholster, P.J. and Erasmus, M. (2023). From garbage to treasure: A review on biorefinery of organic solid wastes into valuable biobased products. Bioresour. Technol. Rep., 24: 101610.
AOAC International. (2005). Official Method 920.160: Acidity (Titrimetric Method). In Official Methods of Analysis of AOAC International (18th ed., pp. 8-10). AOAC International.
Arora, R. and Chandel, A.K. (2023). Unlocking the potential of low FODMAPs sourdough technology for management of irritable bowel syndrome. Food Res. Int., 173: 113425.
Arora, R., Bharti, V.K., Dey, S., 2024. Unlocking the potential of trans-Himalayan sea buckthorn (Hippophae rhamnoides) plants in the green synthesis of silver nanoparticles against drug-resistant foodborne pathogens: a step towards sus
tainable food safety goals. Nano., 19: 4.
Bailey, M.J., Biely, P. and Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. J. Biotech., 23: 257-270.
Bhatt, S., Vaidya, S., Karmacharya, Tamang, A., Manandhar, A. and Neupane, M. (2022). Circumferential intimal tear with thrombosis of right superficial femoral artery due to pene trating injury by bull horn: A case report. Ann. Med. Surg., 4: 103228.
Cheng, N., Barbano, D.M. and Drake, M.A. (2018). Hunter versus CIE color measurement systems for analysis of milk based beverages. J. Dairy Sci., 101: 4891-4905.
Ding, M., Zhang, Y., Li, J. and Pu, K. (2022). Bioenzyme based nanomedicines for enhanced cancer therapy. Nano Converg., 9: 7.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.
Gupta, U., Arora, R. and Kumar, A. (2024). Exploration of horti cultural waste-based bioenzymes for animal wound healing and improving health. In: XXXIX Annual Convention and National Symposium of Indian Society for Study of Animal Reproduction on “Challenges in Enhancing Reproductive Efficiency of Livestock: An Indian Perspective”, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India, November 29-December 1, 2024, pp. 504.
Janarthanan, M., Madhumitha, K., Mani, K., and Ram Shankar Raja, S. (2020). Purification of contaminated water using eco enzyme. IOP Conference Series: Mater. Sci. Eng., 955(1), 1-6. IOP Publishing.
Katan, R.L. and Phaff, H.J. (1959). Purification and properties of a pectic enzyme produced by Saccharomyces fragilis. Arch. Biochem. Biophys., 81: 122-139.
Kurniawan, A., Mustikasari, D., Kurniawan, A., Muntoro, S. J. and Kholishah, N. A. (2024). The preliminary study about physico-chemical property of bio-enzyme produced from orange fruits waste treated with different concentrations of probiotic. Jurnal Ilmu Lingkungan, 22: 861-867.
Lakra, P., Saini, S.K. and Saini, A. (2022). Synthesis, Physio Chemical analysis and applications of bio-enzymes based on fruit and vegetable peels., 9: a670-a681.
Lowry, O.H., Rosebrough, N.J., Farr, A. L. and Randal, L.R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265-275.
Miller, G.J. (1959). Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Anal. Chem., 31: 426- 428.
Mu, X. Shi, S., Hu, X., Gan, X., Jan, Q., Yu, Q., Qu, J. and Li, H. (2024). Gut microbiome and antibiotic resistance genes in plateau model animal (Ochotona curzoniae) exhibit a rela tive stability under cold stress. J. Hazard Mater., 478: 135472
Neupane, K. and Khadka, R. (2019). Production of Garbage Enzyme from Different Fruit and Vegetable Wastes and Evaluation of its JETIR Enzymatic and Antimicrobial
Efficacy. Tribhuvan University J. Microbiol., 6: 113–118. Sethi, S.K., Soni, K., Dhingra, N. and Narula, G.B. (2021). Bringing lab to our home: Bio-enzyme and its multiutility in everyday life. IRJET, 8: 1461-1476.
Toyama, N. and Ogawa, K. (1977). International Course on Biochemical Engineering and Bioconversion. In: Ghose, T. K. (Ed.), Biochemical Engineering Research Centre, IIT, New Delhi, India, pp. 182.
Zhang, H., Jin, J., Wang, X., Han, W., Qin, L., Mao, X., Liu, Z. and Xiang, X. (2024). Bioenzyme–nanoenzyme–chromogen all-in-one test strip for convenient and sensitive detection of malathion in water environment. Sci. Total Environ., 924:
17151.