Effect of surface sterilizing agents on establishment of axenic culture in kinnow mandarin (Citrus deliciosa)
Keywords:
mandarin, sterilization, perennial plants, therapeuticAbstract
The surface sterilization is the most important step prior to initiate tissue culture and can become a critical point in establishment of certain species particularly when the explants was derived from field grown woody perennial plants. Kinnow mandarin (Citrus deliciosa) is a cross, between the king sweet orange (Citrus nobilis) and Willow leaf mandarin (C: deliciosa) developed in California by H.B. Frost. Kinnow fruits have a great therapeutic value due to favourable ratio of K and Na. Kinnow fruits are not only delicious and refreshing but also possess great nutritive value. It contains 10-12% carbohydrate, 0.6-0.8% protein, 0.9-1.1% vitamin A, 0.45-0.55% vitamin C, 0.01- 0.02% thiamine, 0.002 -0.003% riboflavin, 0.25-0.35% calcium, 0.18-0.25% magnesium and 0.028-0.034% iron.Downloads
References
Amin, M.N. and Jaiswal, V.S. (1987). Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tiss. Org. Cult., 9: 235-243.
Kour, K., Bakshi, P. and Kher, R. (2007). Studies on micro- propagation of rough lemon. Indian J Hort. 64 (4): 454-455.
Meghwal, P.R., Sharma, H.C. and Singh, S.K. (2001). Effect of surface sterilizing agents on in vitro culture
establishment of Guava (Psidum guajava L.). Progressive Hort. 33 (1): 101-103.
Karwa, A. (2003). In vitro propagation of citrus reticulate blanco (Nagpur mandarin). Ind. J. Genet. 63 (2): 187 188
Syamal, M.M., Upadhyay, S., and Biswas, B. (2007). in vitro clonal propagation of kegzi lime (Citrus aurantifolia Swingle) Indian J Hort. 64 (1) 84: 86.